M. Gaiffe et al., DESTRUCTION OF SOILS AGGREGATES BY ENZYME S, Comptes rendus de l'Academie des sciences. Serie 2, Mecanique, physique, chimie, sciences de l'univers, sciences de la terre, 319(3), 1994, pp. 331-336
In order to fractionate the soil organic matter into its different com
pounds, it is necessary to previously break completely the structural
units. Physical methods are generally of a limited effect when applied
on strongly aggregated soils; chemical ones need too complex operatio
ns. We investigate in this work a biological method formely used by mi
crobiologists to obtain a bacterial desorption. Hydrolases or antibiot
ics are mixed together with soil samples. In both cases, the cements o
f aggregates are broken by the enzymes. An excellent dispersion is obt
ained when the above mentioned products are simultaneously mechanicall
y dispersed by shaking with small agate beads.