ACCUMULATION OF APOPLASMIC PROTEINS IN BARLEY PRIMARY LEAVES INDUCED BY ALUMINUM AND PATHOGEN TREATMENT

Citation
L. Tamas et al., ACCUMULATION OF APOPLASMIC PROTEINS IN BARLEY PRIMARY LEAVES INDUCED BY ALUMINUM AND PATHOGEN TREATMENT, BIOLOGIA, 52(4), 1997, pp. 585-589
Citations number
21
Categorie Soggetti
Biology
Journal title
BIOLOGIA
ISSN journal
00063088 → ACNP
Volume
52
Issue
4
Year of publication
1997
Pages
585 - 589
Database
ISI
SICI code
0006-3088(1997)52:4<585:AOAPIB>2.0.ZU;2-8
Abstract
Accumulation of the extracellular proteins localized in intercellular spaces of barley primary leaves was examined in aluminium sprayed seed lings. Intercellular washing fluid was obtained from leaves by infiltr ation with 100 mmol.l(-1) citrate-phosphate buffer, pH 2.8 containing 15 mmol.l(-1) mercaptoethanol and proteins analyzed by native polyacry lamide gel electrophoresis. Al induced accumulation patterns of extrac ellular proteins were very similar to that in powdery mildew inoculate d leaves. First detectable differences in comparison with control plan ts were observed after spraying the leaves with 1.0 and 10.0 mmol.l(-1 ) AlCl3. These quantitative differences in accumulation of pathogenesi s related proteins induced with Al treatment were very similar to accu mulation of proteins induced by avirulent races of powdery mildew. Qua litative differences in induction and accumulation of PR have not been detected between barley cultivars Novum and Ricardo.