L. Tamas et al., ACCUMULATION OF APOPLASMIC PROTEINS IN BARLEY PRIMARY LEAVES INDUCED BY ALUMINUM AND PATHOGEN TREATMENT, BIOLOGIA, 52(4), 1997, pp. 585-589
Accumulation of the extracellular proteins localized in intercellular
spaces of barley primary leaves was examined in aluminium sprayed seed
lings. Intercellular washing fluid was obtained from leaves by infiltr
ation with 100 mmol.l(-1) citrate-phosphate buffer, pH 2.8 containing
15 mmol.l(-1) mercaptoethanol and proteins analyzed by native polyacry
lamide gel electrophoresis. Al induced accumulation patterns of extrac
ellular proteins were very similar to that in powdery mildew inoculate
d leaves. First detectable differences in comparison with control plan
ts were observed after spraying the leaves with 1.0 and 10.0 mmol.l(-1
) AlCl3. These quantitative differences in accumulation of pathogenesi
s related proteins induced with Al treatment were very similar to accu
mulation of proteins induced by avirulent races of powdery mildew. Qua
litative differences in induction and accumulation of PR have not been
detected between barley cultivars Novum and Ricardo.