PEROXISOME PROLIFERATOR ACTIVATED RECEPTORS IN ATLANTIC SALMON (SALMO-SALAR) - EFFECTS ON PPAR TRANSCRIPTION AND ACYL-COA OXIDASE ACTIVITY IN HEPATOCYTES BY PEROXISOME PROLIFERATORS AND FATTY-ACIDS

A cDNA fragment which encodes salmon peroxisome proliferator activated receptor gamma (sPPAR gamma) was amplified by PCR from the liver of A tlantic salmon (Salmo salar L.), The fragment was 627 bp long. The seq uence of the amplified PCR product was similar to the PPAR gamma of mo use and hamster. 59% of the bases were identical. Northern blot analys is of salmon liver mRNA showed that the amplified sPPAR gamma fragment hybridised to three specific transcripts of lengths 1.6, 2.4 and 3.3 kb. Clofibric acid and bezafibrate, administered to salmon hepatocytes in culture, resulted in a 1.7-fold increase of the 1.6 kb sPPAR gamma transcript. The activity of acyl-CoA oxidase also increased approx. 1 .7-fold after administration of fibrates. These results indicate that PPAR is an important factor in mediating enzymatic response to fibrate s in fish. (C) 1997 Elsevier Science B.V.