BIOCHEMICAL-GENETIC DIFFERENTIATION IN SOME ROE DEER POPULATIONS OF TUSCANY, CENTRAL ITALY

Polyacrylamide gel electrophoresis was used to assess genetic variabil ity and differentiation at 40 gene loci in 102 roe deer from five samp ling sites in the province of Siena, central Italy. Eight loci proved to be polymorphic in at least one population. Averaged over population s, values of expected heterozygosity (mean He = 0.044, SD = 0.007) and the proportion of polymorphic loci (mean P = 0.135, SD = 0.028) were as high as chose found for roe deer of southern and central Europe. In spite of a moderately high level of gene flow between sites (Nm = 4.7 ), the existence of some degree of genetic structuring in the whole po pulation of Siena could be demonstrated. Demographic events (i.e., res tocking, bottlenecks), rather than genetic isolation were explained to be responsible for the differences in allele frequencies found among populations. The allochthonous origin of roe deer living on Mount Amia ta could be proved by genetic data. The results are discussed in the l ight of historical and demographic information on this species in the province.