A study was undertaken to define the benthic populations, the environm
ental-ecological variability and trophic aspects according to the func
tional feeding groups in the upper reaches of the Nera River, a tribut
ary of the Tiber, in central Italy.; A total of 12 681 individuals was
collected and 83 taxa were identified; 11 proved to be endemic for It
aly, 7 of them typical of the central Apennines. The greatest number o
f taxa (58), and the highest values of diversity were recorded at the
lowest sampling station, 37 km from the source. A dendrogram construct
ed by the UPGMA method based on values of similarity revealed two main
clusters, one identified with crenon (at the source) and the other wi
th rhithron (the downstream stations) fluvial typology. Correspondence
analysis on the environmental matrix parameters confirmed this findin
g. Taxa abundance was evaluated by grouping the individuals collected
into classes using semi-quantitative sampling. The results of trophic-
functional analysis, calculated on the basis of the estimated abundanc
e and tested with statistical methods, agreed with fauna composition a
nalysis clusters for the filtering collector and shredder categories.
The zonal and clinal patterns reveal that there may be a weak upstream
to downstream gradient within a single fluvial zone and almost discon
tinuity in contiguous tracts when the abiotic gradient changes-suddenl
y.