SEQUENCE-ANALYSIS OF A 24-KB CONTIGUOUS GENOMIC REGION AT THE ARABIDOPSIS-THALIANA PFL LOCUS ON CHROMOSOME-1

As part of the European Union program of European Scientist Sequencing Arabidopsis (ESSA), the DNA sequence of a 24,053-bp insert of cosmid clone CC17J13 was determined, The cosmid is located on chromosome 1 at the Pn locus (position 30 cM), Analysis of the sequence and compariso n to public databases predicts seven genes in this area, thus approxim ately one gene every 3.3 kb. Three cDNAs corresponding to genes in thi s region were also sequenced. The homologies and/or possible functions of the (putative) genes are discussed, Proteins encoded by genes in t his region include a polyadenylate-binding protein (PAB-3) and a GTP-b inding protein (Rab7) as well as a novel protein, possibly involved in double-stranded RNA unwinding and apoptosis. Intriguingly, the gene e ncoding the PAB-3 protein, which is very specifically expressed, is fl anked by putative matrix attachment regions. (C) 1997 Federation of Eu opean Biochemical Societies.