The hemolysins of Serpulina hyodysenteriae are active at 27 to 40 degr
ees C and pH 3 to 9 and are unaffected by enzymatic inhibitors. Pore f
ormation was demonstrated by the inhibition of hemolysis with molecule
s of 2.0 to 2.3 nm in diameter and the release of (86)rubidium from er
ythrocytes without hemoglobin release after exposure to native hemolys
in.