U. Haberkorn et al., FDG UPTAKE, TUMOR PROLIFERATION AND EXPRESSION OF GLYCOLYSIS ASSOCIATED GENES IN ANIMAL TUMOR-MODELS, Nuclear medicine and biology, 21(6), 1994, pp. 827-834
To determine the influence of tumor cell proliferation and changes in
the genetic program in malignant cells on the fluorodeoxyglucose (FDG)
uptake we performed PET studies in several animal tumors: spontaneous
mammary fibroadenoma, chemically-induced mammary adenocarcinoma and D
unning prostate adenocarcinoma. The expression of the glucose transpor
ter (GLUT1) and of hexokinase (Hk) was measured using P-32-labeled cDN
A probes and densitometry. Furthermore the proliferative activity was
determined with one-dimensional flow cytometry. The FDG uptake and the
proliferation parameters were not correlated. The normalized amounts
of GLUT and Hk mRNA were lower in spontaneous fibroadenomas and prosta
te tumors than in chemically induced mammary. The FDG uptake was corre
lated to GLUT1 expression with r = 0.83 and to Hk expression with r =
0.77. Multiple regression analysis revealed a relation of FDG uptake t
o GLUT1 and HK with r = 0.87. Our results show that the FDG uptake in
our study was related not to differences in proliferation, but rather
to differences in the transcription of glycolysis associated genes.