O. Johren et al., ANGIOTENSIN-II AT(1A) RECEPTOR MESSENGER-RNA EXPRESSION IS INDUCED BYESTROGEN-PROGESTERONE IN DOPAMINERGIC-NEURONS OF THE FEMALE RAT ARCUATE NUCLEUS, The Journal of neuroscience, 17(21), 1997, pp. 8283-8292
Brain angiotensin II (Ang II) inhibits pituitary prolactin release by
an indirect mechanism requiring stimulation of dopamine formation and
release. We report that [I-125]Sar(1)-Ang II binding to AT(1) receptor
s and AT(1A) receptor mRNA expression increase selectively in the dors
omedial arcuate nucleus of 17 beta-estradiol-primed ovariectomized rat
s after treatment with progesterone. In hormone-treated rats, arcuate
nucleus AT(1A) receptor mRNA expression is associated with tyrosine hy
droxylase-positive neurons. No AT(1A) receptor mRNA was detected in ty
rosine hydroxylase-positive cells of the arcuate nucleus of intact mal
e rats. Conversely, in the anterior pituitary, where local or circulat
ing Ang II stimulates prolactin release, [I-125]Sar(1)-Ang II binding
to AT(1) receptors and AT(1B) receptor mRNA expression are decreased i
n 17 beta-estradiol/progesterone-treated ovariectomized rats. Thus, AT
(1A) receptors in the dorsal arcuate nucleus and AT(1B) receptors in t
he anterior pituitary are regulated inversely by estrogen/progesterone
treatment, supporting the hypothesis of a dual role for brain and pit
uitary Ang II on prolactin release. The colocalization of AT(1A) recep
tor mRNA and tyrosine hydroxylase in neurons of the arcuate nucleus fu
rthermore indicates that within this area central Ang II acts directly
on dopaminergic neurons. These results support the hypothesis that ce
ntral Ang II inhibits pituitary prolactin release indirectly via modul
ation of dopaminergic activity in the arcuate nucleus.