MOLECULAR-GENETIC CHARACTERIZATION OF XRCC4 FUNCTION

Citation
R. Mizuta et al., MOLECULAR-GENETIC CHARACTERIZATION OF XRCC4 FUNCTION, International immunology, 9(10), 1997, pp. 1607-1613
Citations number
20
Categorie Soggetti
Immunology
Journal title
ISSN journal
09538178
Volume
9
Issue
10
Year of publication
1997
Pages
1607 - 1613
Database
ISI
SICI code
0953-8178(1997)9:10<1607:MCOXF>2.0.ZU;2-H
Abstract
XRCC4 is a generally expressed protein of 334 amino acids that is invo lved in the repair of DNA double-strand breaks and in V(D)J recombinat ion, but its function is unknown. In this study, we have used a mutati onal approach and the yeast two-hybrid method to perform an initial ch aracterization of this protein, We show that the XRCC4 protein is loca ted in the nucleus, We also demonstrate that several potential phospho rylation sites are not required for XRCC4 function in a transient V(D) J recombination assay, In addition, we show that XRCC4 forms a homodim er in vivo with the homodimerization domain being located within amino acids 115-204. Finally, we define a core domain of XRCC4 that functio ns in V(D)J recombination and comprises amino acids 18-204, Potential functions of XRCC4 are discussed.