APPLICATION OF IMMUNOHISTOCHEMISTRY AND IN-SITU HYBRIDIZATION FOR DETECTION OF BOVINE CORONAVIRUS IN PARAFFIN-EMBEDDED, FORMALIN-FIXED INTESTINES

Authors
ZHANG Z ANDREWS GA CHARDBERGSTROM C MINOCHA HC KAPIL S
Citation
Z. Zhang et al., APPLICATION OF IMMUNOHISTOCHEMISTRY AND IN-SITU HYBRIDIZATION FOR DETECTION OF BOVINE CORONAVIRUS IN PARAFFIN-EMBEDDED, FORMALIN-FIXED INTESTINES, Journal of clinical microbiology, 35(11), 1997, pp. 2964-2965
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
11
Year of publication
1997
Pages
2964 - 2965
Database
ISI
SICI code
0095-1137(1997)35:11<2964:AOIAIH>2.0.ZU;2-3
Abstract
A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bo vine coronavirus (BCV) reacted with the virus in formalin-fixed intest ines in an immunoperoxidase test. We found an 88% correlation between immunohistochemistry with Z3A5 and in situ hybridization with a BCV nu cleoprotein cDNA probe. MAb Z3A5 reacted with 90 BCV isolates from the United States and was an effective reagent for the diagnosis of BCV.