SEMI QUANTIFICATION OF GIBBERELLINS IN THE ANTHERS OF THERMOSENSITIVEGENETIC MALE-STERILE RICE (ORYZA-SATIVA L. CV PL12)

The levels of endogenous GAs in the anthers of rice (Oryza sativa L., cv. Reimei (normal)) and in those of sterile and fertile plants of a t hermosensitive genetic mate sterile line (Norin PL12; derived from Rei mei) were measured by enzyme-linked immunosorbent assay (ELISA). High levels of GA(4/7) were detected in the anthers of Reimei (normal ferti lity) and fertile (growing under fertile conditions) PL12, while such levels were markedly reduced with increased sterility in PL12, The ant hers of the plants exposed to the sterile conditions contained undevel oped pollens, These results suggest that the occurrence of GA(4/7) was closely related to the expression of the sterility gene and the growt h and/or development of the anther and/or pollen PL12.