A SENSITIVE AND RAPID METHOD FOR MAPPING PROTEIN-BOUND TO DNA BY ATOMIC-FORCE MICROSCOPY

Authors
TANIGAWA M MACHIDA M OKADA T
Citation
M. Tanigawa et al., A SENSITIVE AND RAPID METHOD FOR MAPPING PROTEIN-BOUND TO DNA BY ATOMIC-FORCE MICROSCOPY, Bioscience, biotechnology, and biochemistry, 61(10), 1997, pp. 1751-1753
Citations number
22
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
10
Year of publication
1997
Pages
1751 - 1753
Database
ISI
SICI code
0916-8451(1997)61:10<1751:ASARMF>2.0.ZU;2-O
Abstract
We addressed the strategies of mapping protein binding sites on a DNA fragment by atomic force microscopy (AFM). The protein binding site wa s uniquely mapped by distinguishing two termini of a linear DNA fragme nt. Our simple methods were found very useful to get information on tr anscription regulatory regions by taking advantage of long range and q uick scanning by AFM.