SITE-SPECIFIC ENDONUCLEASES RSPLKI AND RSPLKII FROM RHODOCOCCUS SPECIES LK2 ARE ISOSCHIZOMERS OF SPHI AND BAMHI

Two site-specific endonucleases, RspLKI and RspLKII, have been isolate d and purified to functional homogeneity from the soil bacterium Rhodo coccus species LK2. RspLKI recognizes the 5'-GCATG down arrow C-3' DNA sequence and RspLKII recognizes the 5'-G down arrow 1GATCC-3' sequenc e (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases Sph1 and BamHI, respectively.