Maw. Ruis et Cj. Bayne, EFFECTS OF ACUTE STRESS ON BLOOD-CLOTTING AND YEAST KILLING BY PHAGOCYTES OF RAINBOW-TROUT, Journal of aquatic animal health, 9(3), 1997, pp. 190-195
The effects of different levels of acute stress on blood parameters in
rainbow trout Oncorhynchus mykiss were examined. Experimental fish we
re exposed to a combination of stressors, including handling, confinem
ent, and thermal shock, and were bled 30 s to 30 min later. Control fi
sh were sampled quickly without intentional stress. Two activities of
blood were investigated: (1) whole-blood clotting (coagulation) times
and (2) the ability of heparinized plasma to influence the survival of
yeast soaked in this plasma and subsequently exposed to trout phagocy
tes. The speed of blood clotting increased with increasing levels of s
tress, whereas blood from many resting fish failed to clot. Yeast incu
bated in plasma from stressed fish before exposure to pronephric phago
cytes experienced a greater reduction in colony-forming units (cfus) t
han yeast incubated in plasma from resting fish. Different levels of s
tress did not induce significant differences in reduction of yeast cfu
s. These data support the idea that acute stress may actually stimulat
e primary defense mechanisms rather than suppress them. Furthermore, w
hole-blood clotting times seemed to be reliable indicators of the seve
rity of stressors to which fish were exposed. We postulate that factor
s appearing in blood very quickly, such as catecholamines and specific
plasma proteins, play important roles in both the killing activities
of phagocytes and the blood-clotting process.