URINE PROTEIN-ANALYSIS BY CAPILLARY ELECTROPHORESIS

Increased concentration of proteins in urine as well as abnormal patte rns are seen in many disorders such as various renal disorders and lig ht chain disease. At the wavelength of 214 nm used for detection of th e peptide bond, numerous compounds interfere in the analysis of urinar y proteins. We show that either adsorptive filtration with a wash step or cold ethanol precipitation are two methods which can eliminate man y of the interferences. The wash step is rapid, greatly reduces the in terfering substances, and decreases the effect of sample matrix. Both of these methods yield results comparable to the traditional agarose m ethod. Capillary electrophoresis (CE) is faster and more cost-effectiv e than agarose electrophoresis.