SACCHARIDE MODIFIED SILICA PARTICLES BY ENZYMATIC GRAFTING

The surface of silica particles has been chemically modified with olig o-or poly-(alpha,1-->4)-D-glucopyranose (amylose) chains of various le ngth by covalently attaching maltoheptaose derivatives to the solid su pport and enzymatic polymerization of glucose-phosphate with potato ph osphorylase (E.C. 2.4.1.1) as catalyst. The characterization of the pr oducts by solid-state NMR spectroscopy showed an interesting dependenc e of the linewidth with the grafting density of the glucan chains. The modified silica particles showed chiral discrimination in liquid chro matography.