TSC-36 (FOLLISTATIN-RELATED POLYPEPTIDE) GENE-EXPRESSION IN ESTROGEN-RECEPTOR POSITIVE OSTEOBLASTIC CELL-LINE, CDO7F

Using an osteoblastic cell line (CDO7F) that was selected as an estrog en receptor positive cell by immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) subtractive cDNA clon ing using oligo(dT)(30)-Latex and PCR was performed to isolate a gene that is induced by estrogen. A cDNA clone was isolated and its nucleot ide sequence identified it as TSC-36, previously called follistatin-re lated polypeptide. Northern blot analysis showed that the TSC-36 gene expression was certainly upregulated by estrogen. Tamoxifen also upreg ulated this gene expression. These findings indicate that TSC-36 may b e one of the estrogen-regulated genes in osteoblastic cells.