A SIMPLE HPLC TECHNIQUE FOR ACCURATE MONITORING OF MAMMALIAN-CELL METABOLISM

Authors
STOLL T PUGEAUD P VONSTOCKAR U MARISON IW
Citation
T. Stoll et al., A SIMPLE HPLC TECHNIQUE FOR ACCURATE MONITORING OF MAMMALIAN-CELL METABOLISM, Cytotechnology, 14(2), 1994, pp. 123-128
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Cytotechnology → ACNP
ISSN journal
09209069
Volume
14
Issue
2
Year of publication
1994
Pages
123 - 128
Database
ISI
SICI code
0920-9069(1994)14:2<123:ASHTFA>2.0.ZU;2-4
Abstract
We have developed a simple and accurate isocratic HPLC method, without any prederivatisation, for the determination of glucose, lactate, glu tamine, glutamate, pyrrolidone carboxylic acid and alanine in samples from mammalian cell cultures. The method has been successfully validat ed with enzyme analysis for each of the compounds. Quantification of p yrrolidone carboxylic acid makes the correction for glutamine decrease due to chemical decomposition very simple and accurate, and avoids so me possibly erroneous calculations.