EVALUATION OF MIXED-MODE STATIONARY PHASES IN LIQUID-CHROMATOGRAPHY FOR THE SEPARATION OF CHARGED AND UNCHARGED OLIGOMER-LIKE MODEL COMPOUNDS

Authors
ELEVELD JT CLAESSENS HA AMMERDORFFER JL VANHERK AM CRAMERS CA
Citation
Jt. Eleveld et al., EVALUATION OF MIXED-MODE STATIONARY PHASES IN LIQUID-CHROMATOGRAPHY FOR THE SEPARATION OF CHARGED AND UNCHARGED OLIGOMER-LIKE MODEL COMPOUNDS, Journal of chromatography, 677(2), 1994, pp. 211-227
Citations number
42
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography → ACNP
Volume
677
Issue
2
Year of publication
1994
Pages
211 - 227
Database
ISI
SICI code
Abstract
The possibility to separate charged and uncharged oligomer-like test c ompounds by high-performance liquid chromatography was investigated, u sing mixed-mode (reversed-phase/anion-exchange) stationary phases. In order to examine this possibility, several columns were evaluated for their reversed-phase, anion-exchange and mixed-mode properties using n eutral and charged model compounds. The OmniPac PAX-500, the PRP-X100 and the mixed-phase PLRP-S/PL-SAX columns showed promising results for the separation of real samples.