HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF DIETARY FIBER IN RAW AND PROCESSED CARROTS

An HPLC method was developed to determine dietary fibre in carrots (Da ucus caPota L.). Primary hydrolysis of dietary fibre residue was perfo rmed with 12 M H2SO4 for 2 h at 40 degrees C and secondary hydrolysis with 0.414 M H2SO4 for 3 h at 100 degrees C. For the neutralization st ep prior to injection, AG4-X4 resin (Bio-Rad) was used. Neutral monosa ccharides were separated using an HPX-87P column (Bio-Rad). This metho d was applied for evaluation of the quantitative variation of dietary fibre content in carrots during autoclaving.