IMPROVED TECHNIQUE FOR THE ISOLATION OF STABLE MUTANTS OF ZYMOMONAS-MOBILIS

Authors
ESTEVEZ C MURO C ABATE CM CALLIERI DA SINERIZ F
Citation
C. Estevez et al., IMPROVED TECHNIQUE FOR THE ISOLATION OF STABLE MUTANTS OF ZYMOMONAS-MOBILIS, Folia microbiologica, 42(6), 1997, pp. 562-564
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Folia microbiologica → ACNP
ISSN journal
00155632
Volume
42
Issue
6
Year of publication
1997
Pages
562 - 564
Database
ISI
SICI code
0015-5632(1997)42:6<562:ITFTIO>2.0.ZU;2-O
Abstract
Addition of caffeine to the recovering medium after mutagenesis of Zym omonas mobilis by N-methyl-N-nitrosoguanidine increased l-fold the num ber of auxotrophic mutants obtained. Moreover, while the mutants isola ted without caffeine survived only a few repeated serial transfers on minimal medium supplemented with the required growth factor, 40 % of t hose obtained in the presence of caffeine were stable.