VARIABILITY OF THE NSS PROTEIN AMONG RIFT-VALLEY FEVER VIRUS ISOLATES

Eighteen strains of Rift Valley fever (RVF) virus collected over a per iod of 38 years and isolated from diverse localities in Africa and fro m various hosts (human, animal and arthropod) were investigated by RT- PCR followed by sequencing of the NSs protein coding region. This regi on was chosen to analyse variability because, in contrast to the N pro tein, the NSs protein differs in various phleboviruses and there exist s an EVE virus (clone 13) in which 70% of the NSs ORF is deleted, sugg esting that this sequence is under a weak selective pressure. Sequence data indicated that percentage divergence among isolates ranged from 0 to 9.6% at the nucleotide level and from 0 to 9.5% at the amino acid level. Phylogenetic analysis based on the NSs gene revealed two major lineages: Egyptian and sub-Saharan. This led to the establishment of the relatedness between strains and insights into the NSs protein, the function of which is still undetermined. Alignment of the deduced ami no acid sequences indicated that the cysteine residues are conserved, as are several motifs representing potential phosphorylation sites.