STRUCTURAL EVIDENCE FOR A 2ND SIALIC-ACID BINDING-SITE IN AVIAN INFLUENZA-VIRUS NEURAMINIDASES

Authors
VARGHESE JN COLMAN PM VANDONKELAAR A BLICK TJ SAHASRABUDHE A MCKIMMBRESCHKIN JL
Citation
Jn. Varghese et al., STRUCTURAL EVIDENCE FOR A 2ND SIALIC-ACID BINDING-SITE IN AVIAN INFLUENZA-VIRUS NEURAMINIDASES, Proceedings of the National Academy of Sciences of the United Statesof America, 94(22), 1997, pp. 11808-11812
Citations number
43
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
94
Issue
22
Year of publication
1997
Pages
11808 - 11812
Database
ISI
SICI code
0027-8424(1997)94:22<11808:SEFA2S>2.0.ZU;2-I
Abstract
The x-ray structure of a complex of sialic acid (Neu5Ac) with neuramin idase N9 subtype from A/tern/ AustraIia/G70C/75 influenza virus at 4 d egrees C has revealed the location of a second Neu5Ac binding site on the surface of the enzyme, At 18 degrees C, only the enzyme active sit e contains bound Neu5Ac, Neu5Ac binds in the second site in the chair conformation in a similar way to which it binds to hemagglutinin, The residues that interact with Neu5Ac at this second site are mostly cons erved in avian strains, but not in human and swine strains, indicating that it has some as-yet-unknown biological function in birds.