The BAG-1 protein appears to inhibit cell death by binding to Bcl-2, t
he Raf-l protein kinase, and certain growth factor receptors, but the
mechanism of inhibition remains enigmatic, BAG-1 also interacts with s
everal steroid hormone receptors which require the molecular chaperone
s Hsc70 and Hsp90 for activation, Here we show that BAG-1 is a regulat
or of the Hsc70 chaperone, BAG-1 binds to the ATPase domain of Hsc70 a
nd, in cooperation with Hsp40, stimulates Hsc70's steady-state ATP hyd
rolysis activity similar to 40-fold, Similar to the action of the GrpE
protein on bacterial Hsp70, BAG-1 accelerates the release of ADP from
Hsc70, Thus, BAG-1 regulates the Hsc70 ATPase in a manner contrary to
the Hsc70-interacting protein Hip, which stabilizes the ADP-bound sta
te. Intriguingly, BAG-1 and Hip compete in binding to the ATPase domai
n of Hsc70, Our results reveal an unexpected diversity in the regulati
on of Hsc70 and raise the possibility that the observed anti-apoptotic
function of BAG-1 may be exerted through a modulation of the chaperon
e activity of Hsc70 on specific protein folding and maturation pathway
s.