SEPARATION OF BASIC DRUG ENANTIOMERS BY CAPILLARY-ZONE-ELECTROPHORESIS USING OVOGLYCOPROTEIN AS A CHIRAL SELECTOR

The utilization of crude ovomucoid from chicken egg whites (OMCHI) for the separation of drug enantiomers as a chiral selector in capillary zone electrophoresis (CZE) proved to be unsatisfactory. Because only a bout 10% ovoglycoprotein (OGCHI), which is responsible for chiral reco gnition ability, was included in crude OMCHI, we investigated the util ization of OGCHI as a chiral selector in CZE. To avoid protein adsorpt ion on the wall, two kinds of modified capillaries, a vinyl capillary and a linear polyacrylamide-coated capillary, were employed. Separatio ns of basic drug enantiomers were achieved by optimizing the concentra tions of OGCHI and running buffer, running buffer pH, and the nature a nd content of organic modifier. The present method was successfully ap plied to the assay of eperisone enantiomers. (C) 1997 Elsevier Science B.V.