Hm. Silver et al., RED-CELL VOLUME DETERMINATION USING A STABLE-ISOTOPE OF CHROMIUM, Journal of the Society for Gynecologic Investigation, 4(5), 1997, pp. 254-258
OBJECTIVE: To validate and improve a method of red cell volume determi
nation by use of a stable isotope of chromium. METHODS: Twelve subject
s were sequentially injected with red blood cells labeled with a stabl
e isotope of chromium (Cr-53) and red blood cells labeled with radiois
otopic chromium (Cr-51). Measurement of Cr-53 dilution was by gas chro
matography/mass spectrometry. Measurement of Cr-51 dilution was by gam
ma counter. RESULTS: Comparison of the two methods led to results that
differed on average by 34.5 +/- 45.0 mL (1.8 +/- 2.2%), 0.3 to 3.2%,
95% confidence interval. CONCLUSION: Measurement of red cell volume by
use of a stable isotope of chromium is accurate, with potential appli
cations including measurement is pregnant women and children or other
groups in whom exposure to radioisotopes is undesirable. Copyright (C)
1997 by the Society for Gynecologic Investigation.