(S)-HOMO-AMPA, A SPECIFIC AGONIST AT THE MGLU(6) SUBTYPE OF METABOTROPIC GLUTAMIC-ACID RECEPTORS

Our previous publication (J. Med. Chem. 1996, 39, 3188-3194) described 2-amino-4-(3-hydroxy-5-methylisoxazol-4-yl)butyric acid (Homo-AMPA) a s a highly selective agonist at the mGlu(6) subtype of metabotropic ex citatory amino acid (EAA) receptors. Homo-AMPA has already become a st andard agonist for the pharmacological characterization of mGlu(6) (Tr ends Pharmacol. Sci. Suppl. 1997, 37-39), and we here report the resol ution, configurational assignment, and pharmacology of (S)- (6) and (R )- (7) Homo-AMPA. Using the ''Ugi four-component condensation'', 3-(3- ethoxy-5-methylisoxazol-4-yl)propanal (10) was converted into the sepa rable diastereomeric derivatives of 6 and 7, compounds 12 and 11, resp ectively. Deprotection of 12, in one or two steps, gave extensively ra cemized 6, which was converted in low yield into 6 (99.0% ee) through several crystallizations. 6 (99.7% ee) and 7 (99.9% ee) were finally o btained by preparative chiral HPLC. The configurational assignments of 6 and 7 were based on H-1 NMR spectroscopic studies on 12 and 11, res pectively, and circular dichroism studies on 6 and 7. Values of optica l rotations using different solvents and the chiral HPLC elution order of 6 and 7 supported the results of the spectroscopic configurational assignments. The activities of 6 and 7 at ionotropic EAA (iGlu) recep tors and at mGlu(1-7) were studied. (S)-Homo-AMPA (6) was shown to be a specific agonist at mGlu(6) (EC50 = 58 +/- 11 mu M) comparable in po tency with the endogenous mGlu agonist (S)-glutamic acid (EC50 = 20 +/ - 3 mu M). Although Homo-AMPA did not show significant effects at iGlu receptors, (R)-Homo-AMPA (7), which was inactive at mGlu(1-7), turned out to be a weak N-methyl-D-aspartic acid (NMDA) receptor antagonist (IC50 = 131 +/- 18 mu M).