SITE-SPECIFIC MODIFICATION OF ENZYMATICALLY SYNTHESIZED RNA - TRANSCRIPTION INITIATION AND DIELS-ALDER REACTION

Authors
SEELIG B JASCHKE A
Citation
B. Seelig et A. Jaschke, SITE-SPECIFIC MODIFICATION OF ENZYMATICALLY SYNTHESIZED RNA - TRANSCRIPTION INITIATION AND DIELS-ALDER REACTION, Tetrahedron letters, 38(44), 1997, pp. 7729-7732
Citations number
13
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
44
Year of publication
1997
Pages
7729 - 7732
Database
ISI
SICI code
0040-4039(1997)38:44<7729:SMOESR>2.0.ZU;2-X
Abstract
Initiator nucleotides consisting of an anthracene ring system coupled to the 5'-phosphate of guanosine monophosphate via a polyethylene glyc ol (PEG) linker were chemically synthesized. When added to transcripti on reactions, these initiators were site-specifically incorporated int o RNA transcripts yielding RNA conjugates with a fluorophore selective ly attached to the 5'-end via a flexible linker. Besides sensitive flu orescence detection, the anthracene allowed the convenient modificatio n with maleimides by Diels-Alder reaction. (C) 1997 Elsevier Science L td.