COMPARISON OF VARIOUS TRANSPORT MEDIA ON HUMAN PERIODONTAL-LIGAMENT CELL VIABILITY

The purpose of this study was to determine the ability of various solu tions to maintain human periodontal ligament cell (PDL) viability in v itro, PDL cells were obtained from extracted third molars and premolar s of healthy individuals, These cells were placed into 24-well culture plates containing milk, Save-A-Tooth, Save-A-Tooth supplemented with platelet-derived growth factor-BE (PDGF), or Gatorade at a concentrati on of approximate to 80,000/well. Cells left dry served as negative co ntrols, and cells placed in Eagles' Minimal Essential Medium served as positive controls, At 1, 2, 4, 8, and 12 h, cell viability was evalua ted using an MTS assay and an ELISA plate reader to determine optical density, ANOVA and Student-Newman-Keuls tests indicated that milk and Save-A-Tooth with PDGF are suitable as transport medium for avulsed te eth and that the addition of PDGF to Save-A-Tooth may enhance its abil ity to maintain PDL cell viability, They also suggests that Gatorade w ould be unsuitable as a transport medium.