Dentin permeability and hypersensitivity are both reduced when the den
tinal tubules are occluded. Previous scanning electron microscopic stu
dies showed that Nd:YAG laser could cause melting of dentin and closur
e of exposed dentinal tubules without dentin surface cracking. Therefo
re, the aim of the present study was to evaluate the sealing depth of
Nd:YAG laser on human dentinal tubules. Thirty-six dentin specimens wi
th exposed dentinal tubule orifices were used in this study. Samples w
ere randomly divided into three groups. Groups A and B were lased by N
d:YAG laser at energy of 30 mJ with 10 pulses/s for a stroke along the
dentin surface. Group C was not lased and sewed as a control. Subsequ
ently, group B was frozen in liquid nitrogen and split by a sharp chis
el. Under SEM observation, nonlased specimens showed numerous exposed
dentinal tubule orifices, and lased specimens showed melting of dentin
and closure of exposed dentinal tubule orifices. The sealing depth of
Nd:YAG laser on human dentinal tubules was similar to 4 mu m.