THE PARADIGM THAT ALL OXYGEN-RESPIRING EUKARYOTES HAVE CYTOSOLIC CUZN-SUPEROXIDE DISMUTASE AND THAT MN-SUPEROXIDE DISMUTASE IS LOCALIZED TOTHE MITOCHONDRIA DOES NOT APPLY TO A LARGE GROUP OF MARINE ARTHROPODS

The enzyme superoxide dismutase (SOD), which catalyzes the dismutation of the radical, is present in the cytosol and mitochondria of all oxy gen-respiring eukaryotes. The cytosolic form contains copper and zinc (CuZnSOD),whereas the mitochondrial form contains manganese (MnSOD), T he latter protein is synthesized in the cytosol as a MnSOD precursor, containing an N-terminal mitochondrial-targeting sequence. CuZnSOD is sensitive toward cyanine (CN) and hydrogen peroxide (H2O2), but MnSOD is not. Assays for SOD activity in cytosol from the hepatopancreas of the blue crab, Callinectes sapidus, showed the presence of a CN/H2O2-i nsensitive form of SOD. No CN/H2O2-sensitive CuZnSOD was found. This u nexpected phenomenon was shown to occur in all decapod crustacea (crab s, lobsters, shrimp) examined. The cytosolic and mitochondrial SODs of C. sapidus were purified by means of ion-exchange, size-exclusion, an d reverse-phase HPLC, The cytosolic SOD is a homodimeric protein, whic h exists in a monomer-dimer equilibrium (24 kDa <-> 48 kDa). The prote in contains approximately 1 Mn per subunit. No copper or zinc is prese nt. Amino acid sequence analysis identified the novel cytosolic SOD as a MnSOD precursor with an abnormal mitochondrial-targeting sequence. The mitochondrial SOD of C., sapidus is similar to the MnSOD found in other eukaryotes. N-Terminal amino sequences of mitochondrial and cyto solic blue crab MnSOD differ in several positions. The MnSODs are thus encoded for by two different genes. The paradigm that all eukaryotes contain intracellular CuZnSOD and that MnSOD occurs exclusively in the mitochondria appears not to apply to a large group of marine arthropo ds.