FLUOROMETRIC PROPERTIES OF A 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN - 9-METHYL-BENZO[A]PHENOTHIAZINE INCLUSION COMPLEX IN AQUEOUS-MEDIA - ANALYTICAL USEFULNESS
M. Maafi et al., FLUOROMETRIC PROPERTIES OF A 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN - 9-METHYL-BENZO[A]PHENOTHIAZINE INCLUSION COMPLEX IN AQUEOUS-MEDIA - ANALYTICAL USEFULNESS, Talanta, 44(12), 1997, pp. 2193-2199
The formation of an inclusion complex between 9-methyl-12H-benzo[a]phe
nothiazine (MeBPHT) and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD)
was investigated in aqueous medium. A 12-fold fluorescence emission i
ntensity enhancement was found for the complexed relative to the free
analyte. MeBPHT forms a 1:1 stoichiometry complex with HP-beta-CD. A f
ormation constant of 460 (+/- 100) M-1 was calculated using the Benesi
-Hildebrand method and fluorimetric data. The limit of detection was 7
ng ml(-1) for MeBPHT in the presence of HP-beta-CD instead of 60 ng m
l(-1) in the absence of HP-beta-CD. (C) 1997 Elsevier Science B.V.