FLUOROMETRIC PROPERTIES OF A 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN - 9-METHYL-BENZO[A]PHENOTHIAZINE INCLUSION COMPLEX IN AQUEOUS-MEDIA - ANALYTICAL USEFULNESS

Authors
MAAFI M MAHEDERO MC AARON JJ
Citation
M. Maafi et al., FLUOROMETRIC PROPERTIES OF A 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN - 9-METHYL-BENZO[A]PHENOTHIAZINE INCLUSION COMPLEX IN AQUEOUS-MEDIA - ANALYTICAL USEFULNESS, Talanta, 44(12), 1997, pp. 2193-2199
Citations number
39
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
44
Issue
12
Year of publication
1997
Pages
2193 - 2199
Database
ISI
SICI code
0039-9140(1997)44:12<2193:FPOA2->2.0.ZU;2-S
Abstract
The formation of an inclusion complex between 9-methyl-12H-benzo[a]phe nothiazine (MeBPHT) and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) was investigated in aqueous medium. A 12-fold fluorescence emission i ntensity enhancement was found for the complexed relative to the free analyte. MeBPHT forms a 1:1 stoichiometry complex with HP-beta-CD. A f ormation constant of 460 (+/- 100) M-1 was calculated using the Benesi -Hildebrand method and fluorimetric data. The limit of detection was 7 ng ml(-1) for MeBPHT in the presence of HP-beta-CD instead of 60 ng m l(-1) in the absence of HP-beta-CD. (C) 1997 Elsevier Science B.V.