ASSIGNMENT OF THE HUMAN SEROTONIN 1F RECEPTOR GENE (HTR1F) TO THE SHORT ARM OF CHROMOSOME-3 (3P13-P14.1)

In the present study, we report the chromosomal localization of the hu man 5-HT1F receptor gene (HTR1F) by the analysis of somatic cell hybri ds. Based upon the HTR1F cDNA sequence, a primer set that reacted with human genomic DNA but not mouse or hamster genomic DNA was derived fr om the relatively nonconserved 5'-untranslated and coding region. Usin g monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we lo calized the HTR1F to human chromosome 3. To confirm the localization o n chromosome 3 and to further sublocalize the HTR1F gene, a set of hum an cell hybrids regionally separating chromosome 3 into 7 regions was similarly analysed. Analysis of this regional panel showed that the HT R1F gene was located proximal to the 3p14.1 breakpoint in hybrid APH14 and distal to the breakpoint in 3p13 in hybrid APH13. This localizes the HTR1F gene to human chromosome 3p13-p14.1.