Le. Panin et al., DISTRIBUTION OF THE TOCOPHEROL AND APOLIP OPROTEIN A-I-IMMUNOREACTIVITY IN THE RAT-LIVER CHROMATIN, Biologiceskie membrany, 14(5), 1997, pp. 512-519
[I-125]Labeled lipoproteins (HDL, LDL, and VLDL) are actively uptaken
by the rat liver. The uptake rate by nonparenchymal liver cells was si
gnificantly higher than that by hepatocytes. Analysis of distribution
of the labeled lipoproteins in liver subcellular structures showed the
presence of these compounds in nuclei. The presence of apoA-I-, apoB-
and apoE-immunoreactivity in liver cell nuclei was demonstrated by im
munochemical methods (ELISA and dot immunoassay). The highest apoA-I l
evels were found in the nuclear matrix and transcriptionally active ch
romatin, which considerably exceeded those in the total and transcript
ionally inactive chromatin. Similar distribution pattern was observed
for alpha-tocopherol. The conclusion is made that blood serum lipoprot
eins, first of all HDL, may transport tocopherol and other lipids in c
ell nuclei.