Yp. Zhang et al., AN AUTOCATALYTIC EXPRESSION SYSTEM FOR REGULATED PRODUCTION OF RECOMBINANT PROTEIN IN MAMMALIAN-CELLS, Analytical biochemistry, 252(2), 1997, pp. 286-292
An autocatalytic inducible mammalian expression system was established
. This system is composed of two sets of vectors: one carries a select
able marker gene and the other carries a bicistronic expression unit c
onsisting of a target gene, an internal ribosomal entry site, and a te
tracycline-controlled transactivator gene. Both the selectable marker
and the bicistronic unit are controlled by the tetracycline-responsive
promoter (PhCMV-1). When the two vectors are cotransfected into host
cells, only a single selection round in the absence of tetracycline i
s necessary to generate clones expressing the target gene. The express
ion level is high and easily regulated by tetracycline. Combination wi
th a gene amplification system may allow further enhance the foreign g
ene expression. Because the PhCMV-1 promoter is relatively independen
t of cellular regulation signals, this system is expected to work in a
wide variety of cell types. (C) 1997 Academic Press.