ALLOWANCE FOR KINETICS OF SOLUTE PARTITIONING IN THE DETERMINATION OFRATE CONSTANTS BY AFFINITY-CHROMATOGRAPHY

An improved method is described for evaluation of the dissociation rat e constant for the solute-matrix interaction from affinity chromatogra phic elution profiles in which the flow-rate dependence of the varianc e reflects the combined effects of chemical and partition kinetics. Re sults of a previous investigation of the interaction between saccharid es and concanavalin A immobilized on CPG 3000 are reconsidered in term s of the new procedure, which takes advantage of an analytical express ion to separate the two contributions. Although the revised analysis c onfirms the adequacy of the earlier empirical approach to this problem , it also renders redundant the necessity for the empiricism.