EFFECT OF ADSORBED CONCONOVALIN-A MOLECUL ES ON THE CHARACTERISTICS OF BOUND WATER IN THE HYDRATE SHELL OF DISPERSED SILICA ACCORDING TO H-1-NMR DATA FOR FROZEN AQUEOUS SUSPENSIONS

The experimental technique is presented which allows the investigation of water at the adsorbent-biopolymer interface by record of temperatu re dependencies of H-1 NMR signals during the heating of previously fr ozen water suspensions. It was found that the adsorption of protein co nconovalin A resulted in twofold decrease of strong bound water amount and in 70% Increase of loosely bound one. The mean value of Gibbs ene rgy decreased twice for strong bound water and increased twice for loo sely bound one. The external layer of hydrate shell of silica particle s with adsorbed conconovalin A was organized by protein molecules. Thi s conclusion is based on the observed total increase of loosely bound water amount, as a result, of protein adsorption, although its amount with respect to a protein molecule decreased.