THE EFFECT OF PLATELET-ACTIVATING-FACTOR STRUCTURAL ANALOGS ON SIGNAL-TRANSDUCTION IN MOUSE PERITONEAL NEUTROPHILS AND MACROPHAGES LINE P388D1

Direct and modulate effects of platelet activating factor (PAF), its s tructural analogues and ATP on primary and second processes at periton eal neutrophils and P388D1 cells activation has been studied. The effe ct of compounds was evaluated on changes in Ca2+ transport and generat ion of reactive oxygen species. It was shown, that the synthetic analo gues of MS series interact with PAF receptor, mobilize Ca2+ from thaps igargin-dependent intracellular stores and inhibit Ca2+ response on PA F in both types of cells. Unlike PAF the analogues do not induce the f ormations of reactive oxygen species in neutrophils and inhibit the PM A-induced respiratory burst. The activation of pyrinoreceptor of P388D 1 cells by exogenous ATP does not inhibit PAF induced Ca2+ rise in cyt oplasm, though partly releases Ca2+ from the same store.