NEW PACKING MATERIALS FOR PROTEIN CHROMATOGRAPHY

This review describes new packing materials designed for protein chrom atography, covering advances in base supports and stationary phases. B ase supports are classified according to their chemical composition. S ince most separation media an bead shaped, typical procedures used for their preparation are also presented. In order to provide matrices co mbining improved chemical stability and chromatographic performances, composite materials continue to be developed, including bonded station ary phases, pore composites and mixed carriers. The different approach es to their preparation are described and characteristics that play a major role in the chromatographic process are discussed. Recently intr oduced materials and some of their applications under non-denaturing c onditions in the different chromatographic modes are also presented. ( C) 1997 Elsevier Science B.V.