SEPARATION USED FOR PURIFICATION OF RECOMBINANT PROTEINS

The purification of molecules from recombinant;cells may lie strongly influenced by the molecular biology of gene isolation and expression. At the beginning of the process there may be a demand for information on the minute amounts of proteins and thus for ever increasingly sensi tive techniques. Purification of recombinant proteins can differ from. conventional purifications in several ways, depending on the solubili ty of the protein, occurrence in inclusion bodies, creation of fusion proteins with tags that enable simpler purification. Sometimes a (re)n aturation step is required to get a bioactive protein. On the other ha nd, the techniques used in separation are essentially the same as;for purification from the natural source and environment. (C) 1997 Elsevie r Science B.V.