DETERMINATION OF MINUTE ENZYMATIC-ACTIVITIES BY MEANS OF CAPILLARY ELECTROPHORETIC TECHNIQUES

Capillary electrophoretic analysis of enzymes, co-enzymes, substrates and other chemical species that can be linked to an enzymatic reaction is reviewed with 80 references. Both off-line and on-line assays of m inute enzymatic activities are discussed. In addition to heterogeneous on-line enzyme assays, a special emphasis is given to a newly establi shed on-line technique called electrophoretically mediated microanalys is (EMMA). The basic principle, procedure, and various detection modes of EMMA are discussed. The recent developments in on-line determinati on of various enzyme substrates as well as on-line enzyme kinetic stud ies are also summarized. Some potential future developments in the det ermination of enzymatic activities by means of CE are also presented. (C) 1997 Elsevier Science B.V.