DIRECT PCR OF SMALL GENOMIC DNA FRAGMENTS FROM SERUM

Authors
SANDFORD AJ PARE PD
Citation
Aj. Sandford et Pd. Pare, DIRECT PCR OF SMALL GENOMIC DNA FRAGMENTS FROM SERUM, BioTechniques, 23(5), 1997, pp. 890-892
Citations number
6
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
23
Issue
5
Year of publication
1997
Pages
890 - 892
Database
ISI
SICI code
0736-6205(1997)23:5<890:DPOSGD>2.0.ZU;2-A
Abstract
A simple nod rapid method is described where human genomic DNA suitabl e for PCR was prepared from serum by microwave irradiation. We were ab le to reproducibly amplify single-copy gene sequences up to 442 bp fro m small quantities of serum without the need for DNA extraction. Genot yping results obtained from serum samples ir ere shown to be identical to those derived from purified DNA from the same individuals.