INVESTIGATION OF MONOOXYGENASE OXIDATION ENZYMES IN HUMAN-LYMPHOCYTESAND LIVER EXPOSED TO THE ACTION OF CHLOROPHENOXYHERBICIDES

The activity of benzopyrene hydroxylase (cytochrome P-450IA1) was stud ied in a mitogen-stimulated culture of peripheral blood lymphocytes fr om residents of the South Vietnam area treated with chlorophenoxyherbi cides in the years of American aggression. The population residing in the untreated territory served as the control. The basal and induced b enzopyrene hydroxylase activities, as well as the inducibility ratio w ere determined for each patient using the <<lymphocytic test>>. The co ntent of antipyrine metabolites and their percentage were estimated in the urine of the same patients using the antipyrine test. The compute r processing of data allowed to perform primary analysis of the monoox ygenase system in lymphocytes by groups in order to reveal correlation s between the content of antipyrine metabolites in the urine and the c ytochrome P-450IA1 in blood lymphocytes. The effect of residual amount of 2,3,7,8 tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) on the human mo nooxygenase system is discussed.