ANALYSIS OF A NOVEL ANTIINFLAMMATORY AGENT, METHYLBENZO[B]FURAN-5-YL)-4-CYCLOPROPYLBUTAN-1-ONE (PGV-20229), IN PLASMA MATRICES BY STABLE-ISOTOPE-DILUTION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

A sensitive and selective GC-MS method was developed for the determina tion of low levels of a novel antiinflammatory agent, methylbenzo[b]fu ran-5-yl)-4-cyclopropylbutan-1-one (I), in small volumes of animal pla sma. The method involved the addition of C-13(6)-labeled-I to plasma s amples, followed by a simple liquid-liquid extraction with hexane to i solate the analytes from matrix components. The levels of I in the sam ple extracts were determined by isotope-dilution GC-MS analysis using selected-ion monitoring. The method was linear over three orders of ma gnitude, with a limit of quantitation of 1.8 ng/ml I, using plasma sam ple volumes of 0.1 mi. The method was utilized to determine the pharma cokinetic parameters of I in rats and dogs, following intravenous admi nistration. (C) 1997 Elsevier Science B.V.