VALIDATED ASSAY FOR THE QUANTIFICATION OF ANASTROZOLE IN HUMAN PLASMABY CAPILLARY GAS-CHROMATOGRAPHY NI-63 ELECTRON-CAPTURE DETECTION

An assay was developed for the quantification of anastrozole ethyl)-1, 3-phenylene]bis(2-methylpropiononitrile)] in human plasma using liquid -liquid extraction. Anastrozole and an internal standard chromatograph ed and detected by gas chromatography with electron capture detection, using a combination temperature-pressure program. The range of the as say is 3 to 100 ng/ml. Anastrozole was quantified by comparing its pea k area to that of an internal standard. A cross-validation of this ass ay was also successfully performed between several laboratories. (C) 1 997 Elsevier Science B.V.