SIMULTANEOUS QUANTIFICATION OF CEFOTAXIME, DESACETYLCEFOTAXIME, OFLOXACINE AND CIPROFLOXACINE IN OCULAR AQUEOUS-HUMOR AND IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Hj. Kraemer et al., SIMULTANEOUS QUANTIFICATION OF CEFOTAXIME, DESACETYLCEFOTAXIME, OFLOXACINE AND CIPROFLOXACINE IN OCULAR AQUEOUS-HUMOR AND IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 700(1-2), 1997, pp. 147-153
Citations number
29
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Cefotaxime, given intravenously, is currently used as a broad-spectrum
antibiotic for prophylaxis of intra- and postoperative infections in
ocular lens surgery. A proposed therapeutic and economic alternative i
s the use of orally active fluoroquinolone ofloxacine as prophylactic
agent. A HPLC method was developed for determination of both antibioti
cs in ocular aqueous humor and plasma in order to optimize dosage for
safe surpassing minimal inhibitory concentration in the humor compartm
ent. For plasma determinations a solid-phase extraction procedure was
used with ciprofloxacine as internal standard. Detection limits for di
rect HPLC-analysis of ocular aqueous humor was 0.08 mu g/ml for all co
mpounds, whereas in plasma 0.31 mu g/ml could be determined after soli
d-phase extraction. (C) 1997 Elsevier Science B.V.