DETERMINATION OF SDZ ICM-567 IN BLOOD AND MUSCLE MICRODIALYSIS SAMPLES BY MICROBORE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET AND FLUORESCENCEDETECTION

Fast, simple and accurate methods for the determination of SDZ ICM 567 , the 7-methoxy derivative of tropisetron, in microdialysates have bee n developed. Sampling by microdialysis from freely moving rats in the portal and jugular vein offers a new technology for pharmacokinetic st udies by direct and continuous measurement of unbound drug concentrati ons with time. SDZ ICM 567 can be identified in small sample volumes o f dialysates on a microbore high-performance liquid chromatography col umn-switching system with ultraviolet detection. In addition, determin ation of SDZ ICM 567 by fluorimetric detection has been developed for muscle microdialysates from rats. [C-14]SDZ ICM 567 was used as refere nce substance for the estimation of the amount of substance transferre d through the dialysis membrane. The radioactive measurement (RA) gave the recovery information, whereas the liquid chromatographic method d etected the sum of [C-14]SDZ ICM 567 and dialyzed SDZ ICM 567. (C) 199 7 Elsevier Science B.V.