ANALYSIS OF THE ANTIDIABETIC DRUG ACARBOSE BY CAPILLARY ELECTROPHORESIS

This study describes the derivatization of the pseudooligosaccharide a carbose and its main metabolite, component 2, with 7-aminonaphthalene- 1,3-disulfonic acid (ANDS) in human urine. Their efficient separation was possible by means of capillary zone electrophoresis, using a capil lary tube of fused-silica containing 100 mM triethylammonium phosphate buffer, pH 1.5. On column laser-induced fluorescence allowed the dete ction of the pseudooligosaccharides in human urine in the nanomolar ra nge. With this method, acarbose and component 2 were quantified in hum an urine after application of 300 mg of acarbose. (C) 1997 Elsevier Sc ience B.V.