Sl. Abidi et Tl. Mounts, REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATIONS OFTOCOPHEROLS, Journal of chromatography, 782(1), 1997, pp. 25-32
Citations number
25
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The retention behavior and separation characteristics of five natural
products alpha-, beta-, gamma-, delta-, and zeta(2)-tocopherols and th
eir acetyl derivatives were studied by reversed-phase high-performance
liquid chromatography (HPLC)-fluorescence detection. Investigated sta
tionary phases included an octadecyl polyvinyl alcohol (ODPVA) column,
several octadecylsilica (ODS) columns, and a pentafluorophenylsilica
column. Mobile phases comprised methanol (or acetonitrile) and water a
t variable proportions. Separation factors for beta- and gamma-tocophe
rol isomers and capacity factors of the five tocopherols were determin
ed under various conditions. The beta- and gamma-tocopherols were sepa
rable on ODPVA but not on ODS. However, the beta-gamma pair was resolv
ed with the latter column only as their ester derivatives. HPLC with m
obile phases containing alkanols with carbon atoms greater than 2 favo
red the separation of the beta-gamma pair on ODS and yielded results d
iametrically different but complementary with those obtained with the
ODPVA phase. The combined effects of mobile phases, stationary phases
and antioxidant structures on component separations were delineated. (
C) 1997 Elsevier Science B.V.